Microbial hauberks: composition and function of surface layer proteins in gammaproteobacterial methanotrophs.

ABSTRACT: Many species of proteobacterial methane-consuming bacteria (methanotrophs) form a hauberk-like envelope represented by a surface (S-) layer protein (SLP) matrix. While several proteins were predicted to be associated with the cell surface, the composition and function of the hauberk matrix remained elusive. Here, we report the identification of the genes encoding the hauberk-forming proteins in two gamma-proteobacterial (Type I) methanotrophs, Methylotuvimicrobium buryatense 5GB1 (EQU24_15540) and Methylotuvimicrobium alcaliphilum 20Z^R (MEALZ_0971 and MEALZ_0972). The proteins share 40% amino acid (AA) sequence identity with each other and are distantly related to the RsaA proteins from Caulobacter crescentus (20% AA sequence identity). Deletion of these gen

SUBMITTER: Hamilton R 

PROVIDER: S-EPMC11784148 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature