TolCV1 inhibition by NPPB renders <i>Vibrio vulnificus</i> less virulent and more susceptible to antibiotics.

ABSTRACT: Bacterial efflux pumps play important roles in the antibiotic resistance and excretion of virulence factors. We previously characterized that TolCV1, a component of efflux pumps, plays critical roles in resistance to antibiotics and bile and also RtxA1 toxin secretion of Vibrio vulnificus. In this context, we speculated that TolCV1 blockers would have a dual effect of enhancing susceptibility to antibiotics and suppressing virulence of V. vulnificus. Here, we show that the chloride channel blocker 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) increases susceptibility to antibiotics and suppresses cytotoxicity of V. vulnificus through inhibition of TolCV1. NPPB significantly decreased TolCV1 in V. vulnificus cells by liberating the protein from the cell bod

SUBMITTER: Gong Y 

PROVIDER: S-EPMC11784226 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature