Lipid a remodeling modulates outer membrane vesicle biogenesis by <i>Porphyromonas gingivalis</i>.

ABSTRACT: Outer membrane vesicles (OMVs) are small membrane enclosed sacs released from bacteria which serve as carriers of biomolecules that shape interactions with the surrounding environment. The periodontal pathogen, Porphyromonas gingivalis, is a prolific OMV producer. Here, we investigated how the structure of lipid A, a core outer membrane molecule, influences P. gingivalis OMV production, OMV-dependent TLR4 activation, and biofilm formation. We examined mutant strains of P. gingivalis 33277 deficient for enzymes that alter lipid A phosphorylation and acylation status. The lipid A C4'-phosphatase (lpxF)-deficient strain and strains bearing inactivating point mutations in the LpxF active site displayed markedly reduced OMV production relative to WT. In contrast, str

SUBMITTER: Alaei SR 

PROVIDER: S-EPMC11784228 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature