Positive regulation of a LuxR family protein, MilO, in mildiomycin biosynthesis.

ABSTRACT: Mildiomycin is a representative peptidyl nucleoside antibiotic and was first isolated from Streptoverticillium rimofaciens, which has been used as an important biological agent to control powdery mildew in plants. Despite its importance, the biosynthetic pathways and regulatory mechanisms remain to be fully elucidated. In this study, we identified MilO as a positive pathway-specific regulator of mildiomycin biosynthesis in the heterologous host Streptomyces avermitilis. Gene disruption of milO resulted in almost loss of mildiomycin production, and it was restored to the level comparable to that in the wild-type strain in complemented strain. Overexpression of milO using host native promoter rpsJp, engineered promotor SP44, and kasOp* led to

SUBMITTER: Li Z 

PROVIDER: S-EPMC11784345 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature