Applying a polysaccharide lyase from <i>Stenotrophomonas maltophilia</i> to disrupt alginate exopolysaccharide produced by <i>Pseudomonas aeruginosa</i> clinical isolates.

ABSTRACT: Pseudomonas aeruginosa is considered one of the most challenging, drug-resistant, opportunistic pathogens partly due to its ability to synthesize robust biofilms. Biofilm is a mixture of extracellular polymeric substances (EPS) that encapsulates microbial cells, leading to immune evasion, antibiotic resistance, and thus higher risk of infection. In the cystic fibrosis lung environment, P. aeruginosa undergoes a mucoid transition, defined by overproduction of the exopolysaccharide alginate. Alginate encapsulation results in bacterial resistance to antibiotics and the host immune system. Given its role in airway inflammation and chronic infection, alginate is an obvious target to improve treatment for P. aeruginosa infection. Previously, we demonstrated polysaccharide ly

SUBMITTER: Felton SM 

PROVIDER: S-EPMC11784403 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature