Real-time evaluation of macozinone activity against <i>Mycobacterium tuberculosis</i> through bacterial nanomotion analysis.

ABSTRACT: Novel drugs and improved diagnostics for Mycobacterium tuberculosis (MTB) are urgently needed and go hand in hand. We evaluated the in vitro activity of two benzothiazinone drug candidates (MCZ, PBTZ169; BTZ043) and their main metabolites against MTB using advanced nanomotion technology. The results demonstrated significant reductions in MTB viability within 7 h, indicating the potential for rapid, precise antibiotic susceptibility testing based on a phenotypic read-out in real time. PBTZ169 and H₂-PBTZ169 achieved 100% separation between the susceptible H37Rv and a resistant dprE1 mutant strain NTB1. These findings support nanomotion technology's potential for faster antibiotic susceptibility testing of novel MTB drug candidates targeting the DprE1 enzyme t

SUBMITTER: Vocat A 

PROVIDER: S-EPMC11784433 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature