Genetic identification of acetyl-CoA synthetases involved in acetate activation in <i>Haloferax mediterranei</i>.

ABSTRACT: Acetate/acetyl-CoA interconversion is an interesting metabolic node, primarily catalyzed by a set of various enzymes in prokaryotes. Haloferax mediterranei is a promising haloarchaeaon, capable of utilizing acetate as a sole carbon source for biosynthesis of high value-added products. Here, we have reported the key enzymes that catalyzed acetate activation in H. mediterranei. Based on bioinformatic and transcript analysis, thirteen possible candidate genes were screened. Simultaneous deletion of eleven genes led to a mutant strain (named as Δ11) that failed to grow on acetate. Gene complementation in Δ11 revealed six AMP-ACS (encoded by HFX_0870, HFX_1242, HFX_1451, HFX_6342, HFX_5131, and HFX_1643) and one ADP-ACS (encoded by HFX_0998) to be functional in acetate activation.

SUBMITTER: Mitra R 

PROVIDER: S-EPMC11784441 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature