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Persistence of human Aichi virus infectivity from raw surface water to drinking water.


ABSTRACT: Human Aichi virus 1 (AiV-1) is a water- and food-borne infection-associated picornavirus that causes gastroenteritis in humans. Recent studies on environmental waters showed a high frequency and abundance of AiV-1, suggesting that it might be an appropriate indicator of fecal contamination. We screened 450 surface and drinking water samples from a Tunisian drinking water treatment plant (DWTP) and the Sidi Salem dam for AiV-1 by real time reverse transcriptase PCR (RT-qPCR). The persistence of infectious particles was evaluated using an integrated cell culture approach coupled with quantitative molecular detection (ICC-RT-qPCR). In all, 85 (18.9%) water samples were positive for AiV-1 with viral loads ranging from 0.47 to 11.62 log10 cp/L and a median of 4.97 log10 cp

SUBMITTER: Sdiri-Loulizi K 

PROVIDER: S-EPMC11784453 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature