Impact of infusion conditions and anesthesia on CSF tracer dynamics in mouse brain.

ABSTRACT: Tracer imaging has been instrumental in mapping the brain's solute transport pathways facilitated by cerebrospinal fluid (CSF) flow. However, the impact of tracer infusion parameters on CSF flow remains incompletely understood. This study evaluated the influence of infusion location, rate, and anesthetic regimens on tracer transport using dynamic contrast-enhanced MRI with Gd-DTPA as a CSF tracer. Infusion rate effects were assessed by administering Gd-DTPA into the cisterna magna (ICM) at two rates under isoflurane anesthesia. Anesthetic effects were evaluated by comparing transport patterns between isoflurane and ketamine/xylazine (K/X) anesthesia at the slower rate. Gd-DTPA transport was also examined after lateral ventricle (ICV) infusion, the primary site of CSF production. The result

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC11785030 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature