The E2F4 transcriptional repressor is a key mechanistic regulator of colon cancer resistance to <i>irinotecan</i> (CPT-11).

ABSTRACT:

Background

Colorectal carcinomas (CRCs) are seldom eradicated by cytotoxic chemotherapy. Cancer cells with stem-like functional properties, often referred to as "cancer stem cells" (CSCs), display preferential resistance to several anti-tumor agents used in cancer chemotherapy, but the molecular mechanisms underpinning their selective survival remain only partially understood.

Methods

In this study, we used Transcription Factor Target Genes (TFTG) enrichment analysis to identify transcriptional regulators (activators or repressors) that undergo preferential activation by chemotherapy in CRC cells with a "bottom-of-the-crypt" phenotype (EPCAM⁺/CD44⁺/CD166⁺; CSC-enriched) as compared to CRC cells with a "top-of-th

SUBMITTER: Matsubara J 

PROVIDER: S-EPMC11785039 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature