Distinct cell types along thick ascending limb express pathways for monovalent and divalent cation transport.

ABSTRACT: Kidney thick ascending limb cells reabsorb sodium, potassium, calcium, and magnesium and contribute to urinary concentration. These cells are typically viewed as of a single type that recycles potassium across the apical membrane and generates a lumen-positive transepithelial voltage driving calcium and magnesium reabsorption, although variability in potassium channel expression has been reported. Additionally, recent transcriptomic analyses suggest that different cell types exist along this segment, but classifications have varied and have not led to a new consensus model. We used immunolocalization, electrophysiology and enriched single nucleus RNA-Seq to identify thick ascending limb cell types in rat, mouse and human. We identified three major TAL cell types defined by expression of po

SUBMITTER: Demirci H 

PROVIDER: S-EPMC11785040 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature