DNAHX: a novel, non-motile dynein heavy chain subfamily, identified by cryo-EM endogenously.

ABSTRACT: Ciliogenesis and cilia motility rely on the coordinated actions of diverse dyneins, yet the complexity of these motor proteins in cilia has posed challenges for understanding their specific roles. Traditional evolutionary analyses often overlook key family members due to technical limitations. Here, we present a cryo-EM-based, bottom-up approach for large-scale, de novo protein identification and functional prediction of endogenous axonemal dynein complexes. This approach led to the identification of a novel dynein heavy chain subfamily (XP_041462850), designated as DNAHX, from sea urchin sperm. Phylogenetic analysis indicates that DNAHX branches from the outer-arm dynein alpha chain during evolution and is found in specific animal lineages with external fertilization. DNAHX contain

SUBMITTER: Chai P 

PROVIDER: S-EPMC11785096 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature