Solution conformational differences between conventional and CENP-A nucleosomes are accentuated by reversible deformation under high pressure.

ABSTRACT: Solution-based interrogation of the physical nature of nucleosomes has its roots in X-ray and neutron scattering experiments, including those that provided the initial observation that DNA wraps around core histones. In this study, we performed a comprehensive small-angle scattering study to compare canonical nucleosomes with variant centromeric nucleosomes harboring the histone variant, CENP-A. We used nucleosome core particles (NCPs) assembled on an artificial positioning sequence (Widom 601) and compared these to those assembled on a natural α-satellite DNA cloned from human centromeres. We establish the native solution properties of octameric H3 and CENP-A NCPs using analytical ultracentrifugation (AUC), small-angle X-ray scattering (SAXS), and contrast variation small-angle neutron sc

SUBMITTER: Gupta K 

PROVIDER: S-EPMC11785105 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature