Single-cell differential detergent fractionation for detection of cytokeratin 8 proteoforms.

ABSTRACT: Simultaneous profiling of proteoforms and nucleic acids at the single-cell level, i.e., multi omics, directly links the central dogma. However, current single-cell approaches are limited in their ability to identify proteoforms while preserving the nucleus for further analysis. This limitation is especially pronounced in proteins where their proteoforms present diverse biological functions such as cytokeratin 8 (CK8), which, while commonly known for its structural role, is also involved in several diseases. Here, we present a single-cell western blot (scWB) integrated with differential detergent fractionation (DDF) to selectively solubilize and separate CK8 proteoforms while preserving nuclear integrity for subsequent nucleus-based assays. We report on assay development, including screenin

SUBMITTER: Khartchenko AF 

PROVIDER: S-EPMC11785136 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature