DMEM and EMEM are suitable surrogate media to mimic host environment and expand leptospiral pathogenesis studies using <i>in vitro</i> tools.

ABSTRACT: Pathogenic Leptospira species can survive and thrive in a wide range of environments. Distinct environments expose the bacteria to different temperatures, osmolarities, and amounts and sources of nutrition. However, leptospires are mostly cultured, in a laboratory setting under in vitro conditions that do not reflect natural environments. This constraint on laboratory cultures limits the applicability of in vitro studies to the understanding of even simple pathogenic processes. Here we report, investigate, and identify a medium and conditions that mimic the host environment during leptospirosis infection, expanding the available in vitro tools to evaluate leptospiral pathogenesis. We quantified genome-wide gene expression of pathogenic Leptospira interrogans<

SUBMITTER: Garcia LE 

PROVIDER: S-EPMC11785191 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature