Single-cell transcriptomic profiling of heart reveals ANGPTL4 linking fibroblasts and angiogenesis in heart failure with preserved ejection fraction.

ABSTRACT:

Introduction

Despite the high morbidity and mortality, the effective therapies for heart failure with preserved fraction (HFpEF) are limited as the poor understand of its pathophysiological basis.

Objective

This study was aimed to characterize the cellular heterogeneity and potential mechanisms of HFpEF at single-cell resolution.

Methods

An HFpEF mouse model was induced by a high-fat diet with N-nitro-L-arginine methyl ester. Cells from the hearts were subjected to single-cell sequencing. The key protein expression was measured with Immunohistochemistry and immunofluorescence staining.

Results

In HFpEF hearts, myocardial fibroblasts exhibited higher levels of fibrosis. Furthermore, an increased number of fibroblasts differentiated into high-metabolism and high-

SUBMITTER: Li G 

PROVIDER: S-EPMC11785561 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature