HnRNPR-mediated UPF3B mRNA splicing drives hepatocellular carcinoma metastasis.

ABSTRACT:

Introduction

Abnormal alternative splicing (AS) contributes to aggressive intrahepatic invasion and metastatic spread, leading to the high lethality of hepatocellular carcinoma (HCC).

Objectives

This study aims to investigate the functional implications of UPF3B-S (a truncated oncogenic splice variant) in HCC metastasis.

Methods

Basescope assay was performed to analyze the expression of UPF3B-S mRNA in tissues and cells. RNA immunoprecipitation, and in vitro and in vivo models were used to explore the role of UPF3B-S and the underlying mechanisms.

Results

We show that splicing factor HnRNPR binds to the pre-mRNA of UPF3B via its RRM2 domain to generate an exon 8 exclusion truncated splice variant UPF3B-S. High expression of UPF3B-S is correlated with tumor meta

SUBMITTER: Wang H 

PROVIDER: S-EPMC11785583 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature