Exploring the impacts of senescence on implantation and early embryonic development using totipotent cell-derived blastoids.

ABSTRACT:

Introduction

Advanced maternal age is associated with reduced implantation and pregnancy rates, yet the underlying mechanisms remain poorly understood, and research models are limited.

Objectives

Here, we aim to elucidate the impacts of senescence on implantation ability by employing blastoids to construct a novel research model.

Methods

We used a novel three-dimensional system with totipotent blastomere-like cells (TBLCs) to construct TBL-blastoids and established senescence-related embryo models derived from oxidative stress-induced TBLCs.

Results

Morphological and transcriptomic analyses revealed that TBL-blastoids exhibited characteristic blastocyst morphology, cell lineages, and a higher consistency in developmental rate. TBL-blastoids demonstrated the abi

SUBMITTER: Luo Y 

PROVIDER: S-EPMC11785586 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature