Histamine and TH2 cytokines regulate the biosynthesis of cysteinyl-leukotrienes and expression of their receptors in human mast cells.

ABSTRACT:

Introduction

In skin lesions of atopic dermatitis (AD), a chronic inflammatory skin disease, mast cells beyond other immune cells are present in increasing numbers. Upon activation, mast cells release a plethora of mediators, in particular histamine and leukotrienes, as well as chemokines and cytokines, which modulate the immune response of cells in their microenvironment and may influence mast cells in an autocrine loop. This study investigated the effects of histamine and TH2 cytokines on the biosynthesis of cysteinyl leukotrienes (CysLTs) as well as CysLT receptor expression on human mast cells from healthy volunteers and patients with AD.

Methods

Human mast cells were generated from CD34+ progenitor cells from peripheral blood. The cultured mast cells were stimulated wit

SUBMITTER: Gehlhaar P 

PROVIDER: S-EPMC11785601 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature