Double-negative T cells with a distinct transcriptomic profile are abundant in the peripheral blood of patients with breast cancer.

ABSTRACT:

Background

Double-negative T (DNT) cells comprise a distinct subset of T lymphocytes that have been implicated in immune responses. The aim of this study was to characterize the peripheral DNT population in breast cancer (BC) patients.

Methods

DNT cells were isolated from the peripheral blood samples of BC patients and healthy controls by flow cytometry. The sorted DNT cells were analyzed by the Smart-seq2 for single-cell full-length transcriptome profiling. The differentially expressed genes (DEGs) between the BC and control groups were screened and functionally annotated by Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses using R. The protein-protein interaction (PPI) network of the DEGs was constructed using the CytoHubba a

SUBMITTER: Zhu HR 

PROVIDER: S-EPMC11785702 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature