Glucomannan engineering highlights roles of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls.

ABSTRACT: Widely found in most plant lineages, β-mannans are structurally diverse polysaccharides that can bind to cellulose fibrils to form the complex polysaccharide architecture of the cell wall. How changes in polysaccharide structure influence its cell wall solubility or promote appropriate interaction with cellulose fibrils is poorly understood. Glucomannan backbones acquire variable patterns of galactosyl substitutions, depending on plant developmental stage and species. Here, we show that fine-tuning of galactosyl modification on glucomannans is achieved by the differing acceptor recognition of mannan α-galactosyltransferases (MAGTs). Biochemical analysis and ¹³C solid-state　nuclear magnetic resonance spectroscopy of Arabidopsis with cell wall glucomannan engineered by MAGTs revea

SUBMITTER: Yoshimi Y 

PROVIDER: S-EPMC11785759 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature