Low titre of agroinoculum with prolonged incubation period and low auxin concentration in the regeneration media are the key to high frequency of transformation in climate-resilient Aus-type rice genotype Nagina 22.

ABSTRACT: Developing an efficient and reproducible regeneration protocol holds paramount significance for advancing genetic transformation technologies in rice, facilitating their utilisation in crop improvement. Nagina 22 (N22), a climate-resilient Aus-type rice genotype known for its tolerance against multiple stresses, lacks a standardised transformation protocol, limiting its utilisation as a background for genetic transformation. This study reports, for the first time, a highly efficient transformation and regeneration protocol for N22 using a CRISPR/Cas9 vector. Mature seeds were used to induce embryogenic calli on CHU(N6)-based callus induction media (CIM) with varying concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D). The highest callus induction efficiency (~ 94%) was achieved

SUBMITTER: Kumar A 

PROVIDER: S-EPMC11785844 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature