Clinicopathologic Effects of Xenogeneic GvHD Induced by Adoptively Transferred Human-Derived T Cells in Severely Immunodeficient Mice.

ABSTRACT:

Background

Xenogeneic graft-versus-host disease (xGvHD) is an inevitable confounder of preclinical evaluation of adoptive immunotherapies on tumor-bearing immunodeficient mouse models. This study was designed to appraise the clinical and histopathological effects caused by xGvHD in severely immunodeficient mice considering the T cell dosage.

Methods

Fifty NOG mice underwent intraperitoneal injection of three different doses of human-derived total T cells, a high dose of CD8⁺T cells, or vehicle (as control). Clinical and histopathological status of the study subjects were evaluated and compared according to scoring systems.

Results

In mice receiving higher doses of total T cells, the clinical severity of xGvHD was greater. However, recipients of CD8⁺

SUBMITTER: Ashraf H 

PROVIDER: S-EPMC11786209 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature