Fibrous corona is reduced in cancer cell lines that attenuate microtubule nucleation from kinetochores.

ABSTRACT: Most cancer cells show increased chromosome missegregation, known as chromosomal instability (CIN), which promotes cancer progression and drug resistance. The underlying causes of CIN in cancer cells are not fully understood. Here we found that breast cancer cell lines show a reduced kinetochore localization of ROD, ZW10, and Zwilch, components of the fibrous corona, compared with non-transformed breast epithelial cell lines. The fibrous corona is a structure formed on kinetochores before their end-on attachment to microtubules and plays a role in efficient kinetochore capture and the spindle assembly checkpoint. The reduction in the fibrous corona was not due to reduced expression levels of the fibrous corona components or to a reduction in outer kinetochore components. Kinetochore locali

SUBMITTER: Ishikawa Y 

PROVIDER: S-EPMC11786318 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature