A comparative analysis of sequence composition in different lots of a phage display peptide library during amplification.

ABSTRACT:

Background

To develop efficient selection strategies and improve the discovery of promising ligands, it is highly desirable to analyze the sequence composition of naïve phage display libraries and monitor the evolution of their peptide content during successive rounds of amplification. In the current study, we performed a comparative analysis of the compositional features in different lots of the same naïve phage display library and monitored alterations in their peptide compositions during three rounds of amplification.

Methods

We conducted three rounds of duplicate serial amplification of two different lots of the Ph.D.™-12 phage display library. DNA from the samples was subjected to Next-Generation Sequencing (NGS) using an Illumina platform. The NGS datasets underwent a

SUBMITTER: Sinkjaer AW 

PROVIDER: S-EPMC11786364 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature