Xanthomonas citri subsp. citri requires a polyketide cyclase to activate the type III secretion system for virulence.

ABSTRACT:

Background

Xanthomonas citri subsp. citri is the causal agent of citrus canker, which causes substantial losses in citrus production. Here, we report the role of a polyketide cyclase (PKC) on the virulence in X. citri subsp. citri.

Methods

The structure of PKC was precisely predicted using Alphafold3. Promoter GUS fusion constructs and real-time quantitative reverse transcription (qRT-PCR) were employed to study the pattern of expression of the polyketide gene. A deletion mutation was created to explore the role of PKC in virulence and metabolic change.

Results

The PKC was determined to have a signal peptide, a START/RHO_alpha_C/PITP/Bet_v1/CoxG/CalC (SRPBCC) domain, and a GyrI-like small molecule binding domain. The expression of the PKC gene was induced in planta, a

SUBMITTER: Zhu S 

PROVIDER: S-EPMC11786466 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature