Spatial profiling of endoplasmic reticulum stress markers in tumor associated cells predicts patient outcomes in pancreatic cancer.

ABSTRACT:

Introduction

The impact of endoplasmic reticulum (ER) stress in tumor-associated cells, such as cancer associated fibroblasts (CAFs), immune cells and endothelial cells, on patient outcomes in clinical specimens have not been examined. For the first time, we characterized the expression and spatial locations of ER stress markers, BiP and CHOP, in tumor-associated cells and assessed their prognostic significance in a panel of pancreatic ductal adenocarcinoma (PDAC) patient samples.

Methods

Multiplex immunofluorescence was performed on tumor microarrays and images were analyzed using HALO AI software.

Results

BiP and CHOP were upregulated in CAFs and endothelial cells in PDAC sections relative to non-neoplastic pancreas sections. High BiP expression in CAFs and endothel

SUBMITTER: Porter G 

PROVIDER: S-EPMC11786694 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature