An enzyme-activated loop primer probe LAMP method based on a new SNP site in the group_17537 gene for rapid on-site detection of Salmonella Pullorum.

ABSTRACT: Pullorum disease (PD) is a widespread disease that causes significant economic losses within the poultry industry of developing countries. An effective strategy for its prevention and control involves the implementation of decontamination procedures utilizing highly specific on-site detection techniques. In this study, a single-nucleotide polymorphism (SNP) site within the group_17537 gene of Salmonella enterica serovar Gallinarum biovars Pullorum (S. Pullorum) was found by using bioinformatics tools. The prevalence of this SNP among 165 strains of S. Pullorum was determined to exceed 96.3 %. The SNP exhibited a specificity rate greater than 99.9 %, with only 0.08 % detected among 2490 non-target Salmonella strains. It can be concluded that this SNP can be employed to distinguish S. Pullor

SUBMITTER: Xu X 

PROVIDER: S-EPMC11786731 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature