Cysteine import via the high-affinity GSH transporter Hgt1 rescues GSH auxotrophy in yeast.

ABSTRACT: Glutathione (GSH) is an abundant thiol-containing tripeptide that functions in redox homeostasis, protein folding, and iron (Fe) metabolism. In Saccharomyces cerevisiae, GSH depletion leads to increased sensitivity to oxidants and other toxic compounds, disruption of iron-sulfur (Fe-S) cluster biogenesis, and eventually cell death. GSH pools are supplied by intracellular biosynthesis and GSH import from the extracellular environment. Consequently, in GSH-depleted growth media, deletion of the gene encoding the first enzyme in the GSH biosynthetic pathway (GSH1) is lethal in yeast. At the other extreme, GSH overaccumulation via overexpression of the high-affinity GSH transporter Hgt1 is also toxic to cells, leading to reductive stress. Here, we engineered a yeast strain that combines gsh1 d

SUBMITTER: McGee CC 

PROVIDER: S-EPMC11786745 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature