ISCOM-type matrix from beta-escin and glycyrrhizin saponins.

ABSTRACT:

Background and aims

Nanotechnology provides the opportunity for construction of modern transport devices such as nanoparticles for a variety of applications in the field of medicine. A novel experimental protocol for the formation of saponin-cholesterol-phospholipid nanoparticles of vesicular structure has been developed and applied to prepare stable nanoparticles using escin or glycyrrhizin as saponins.

Methods

The methods for nanoparticle construction include a sonication at 90 °C of the initial mixture of components, followed by an additional sonication on the next day for incorporation of an additional amount of cholesterol, thus forming stable unilamellar vesicles. Tests and assays for cell viability, erythrocyte hemolysis, flow cytometry, and fluorescent microscopy ana

SUBMITTER: Petkov V 

PROVIDER: S-EPMC11786834 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature