The profiles of immunosuppressive microenvironment in the Lauren intestinal-type gastric adenocarcinoma.

ABSTRACT:

Background

Gastric adenocarcinoma (GAC), particularly the Lauren intestinal-type GAC (IGAC), leads to significant mortality in China due to the limited effectiveness of current treatments. This study aims to investigate the mechanisms of immune suppression in IGAC to identify potential targets for enhancing immunotherapy outcomes.

Methods

Performing an extensive collection and re-analysis of single-cell RNA sequencing (scRNA-seq) of tumor tissues and the corresponding noncancerous mucosae from 15 Chinese patients diagnosed with IGAC, we identified cell subpopulations involved in immune suppression within the tumor microenvironment (TME). We further validated our findings using spatially resolved transcriptomics (SRT), immunofluorescence (IF), and flow cytometry (FCM) on tiss

SUBMITTER: Wang Q 

PROVIDER: S-EPMC11787096 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature