Natural occurrence of episomal banana streak GF virus species infecting the GI-tagged banana cv. Virupakshi in India.

ABSTRACT: Banana streak disease was identified as a new threat to banana cultivar Virupakshi (syn: Hill banana, AAB), registered under Geographical Indications (G.I. 124) in India. PCR and rolling circle amplification (RCA) of infected leaf samples revealed the presence of banana streak GF virus (BSGFV) as the causative agent of streak symptoms. RCA and sequence analysis identified an episomal BSGFV variant (BSGFV-IN; MW389538) along with the full-length BSGFV genome (BSGFV-IN1; PP134844). The shorter BSGFV-IN genome measured 6590 bp with a deletion of 673 bp, while the full-length BSGFV-IN1 genome was 7263 bp. Sequencing of the cloned fragments using MinION nanopore sequencer further confirmed these findings, with both isolates showing > 99% sequence similarity to the BSGFV (AY493509) from Ecuador.

SUBMITTER: Selvarajan R 

PROVIDER: S-EPMC11787140 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature