The Vitamin D-Sirt1/PGC1α Axis Regulates Bone Metabolism and Counteracts Osteoporosis.

ABSTRACT:

Background

Objective: Vitamin D insufficiency is a major contributor to osteoporosis. This study aimed to elucidate the mechanisms by which the vitamin D-Sirt1/PGC1α axis regulates bone metabolism and counteracts osteoporosis induced by active vitamin D insufficiency.

Methods

Mouse models including Sirt1 transgenic (Sirt1^Tg), Cyp27b1^+/- (active vitamin D deficient), and compound Sirt1^TgCyp27b1^+/- mice were utilized. Bone parameters were assessed by radiography, micro-CT, histology, and immunohistochemistry. In vitro studies used bone marrow-derived mesenchymal stem cells (BM-MSCs). Gene and protein expression were analyzed by RT-PCR and Western blotting. Chromatin immunoprecipitation and luciferase assays investigated transcription

SUBMITTER: Yang C 

PROVIDER: S-EPMC11787469 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature