Development of a High-Throughput Platform for Quantitation of Histone Modifications on a New QTOF Instrument.

ABSTRACT: Histone post-translational modifications (PTMs) regulate gene expression patterns through epigenetic mechanisms. The five histone proteins (H1, H2A, H2B, H3, and H4) are extensively modified, with over 75 distinct modification types spanning more than 200 sites. Despite strong advances in mass spectrometry (MS)-based approaches, identification and quantification of modified histone peptides remains challenging because of factors, such as isobaric peptides, pseudo-isobaric PTMs, and low stoichiometry of certain marks. Here, we describe the development of a new high-throughput method to identify and quantify over 150 modified histone peptides by LC-MS. Fast gradient microflow liquid chromatography and variable window sequential windows acquisition of all theoretical spectra data-independent

SUBMITTER: Zahn E 

PROVIDER: S-EPMC11787651 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature