Annotation and characterization of immunoglobulin loci and CDR3 polymorphism in water buffalo (<i>Bubalus bubalis</i>).

ABSTRACT:

Introduction

Immunoglobulin (Ig) refers to the nomenclature for all antibody proteins produced by B lymphocytes. The genetic locus encoding Ig is critical for vertebrate humoral immune responses and diverse antibody repertoires. Despite the critical role of buffaloes as livestock and their significance in disease transmission, the Ig loci of this species have not been thoroughly annotated. This study aimed to systematically characterize the Ig loci in buffaloes and their unique features, providing a foundation for understanding buffalo immune function.

Methods

The genomic assembly of Murrah buffalo (NDDB_SH_1) was analyzed to annotate Ig loci. Annotation criteria included functional motifs, RSS sequences, and structural features of V, D, J, and C genes. The CDR3 repertoires

SUBMITTER: Deng Y 

PROVIDER: S-EPMC11788136 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature