<i>Brucella suis</i> Δ<i>mapB</i> outer membrane vesicles as an acellular vaccine against systemic and mucosal <i>B. suis</i> infection.

ABSTRACT:

Introduction

Swine brucellosis, caused by Brucella suis, is a worldwide infectious zoonotic disease. Currently, there are no available human or porcine vaccines to protect against B. suis infection, which is primarily acquired through the mucosa. We recently described B. suis MapB, the homologous protein of TamB, the inner membrane component of the TAM system. Our findings indicate that MapB is involved in bacterial cell envelope homeostasis. In this study, we characterize the outer membrane vesicles (OMVs) of B. suis 1330 (wt) and those of B. suis ΔmapB (ΔmapB) mutant strain and evaluate their vaccine potential in mice.

Methods

OMVs were isolated using the ultracentrifugation method and characterized through electron microsco

SUBMITTER: Gonzalez FM 

PROVIDER: S-EPMC11788153 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature