Cell clone selection-impact of operation modes and medium exchange strategies on clone ranking.

ABSTRACT: Bioprocessing has been transitioning from batch to continuous processes. As a result, a considerable amount of resource was dedicated to optimising strategies for continuous production. However, the focus has been on developing a suitable and scalable perfusion strategy with little attention given to the selection of optimal cell clones. Cell line development and lead clone selection are critical to bioprocess development. The screening and selection process is typically performed in stages. Microwell plates (MWP) are used to narrow down the number of clone candidates, which will undergo further selective screening in progressively larger small-scale bioreactors (12 mL-3 L) to identify the top clone for GMP production. Perfusion mode is typically applied at bench-scale for optimisation pur

SUBMITTER: Dorn M 

PROVIDER: S-EPMC11788354 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature