Assembly landscape of the complete B-repeat superdomain from Staphylococcus epidermidis strain 1457.

ABSTRACT: The accumulation-associated protein (Aap) is the primary determinant of Staphylococcus epidermidis device-related infections. The B-repeat superdomain is responsible for intercellular adhesion that leads to the development of biofilms occurring in such infections. It was recently demonstrated that Zn-induced B-repeat assembly leads to formation of functional amyloid fibrils, which offer strength and stability to the biofilm. Rigorous biophysical studies of Aap B-repeats from S. epidermidis strain RP62A revealed Zn-induced assembly into stable, reversible dimers and tetramers, prior to aggregation into amyloid fibrils. Genetic manipulation is not tractable for many S. epidermidis strains, including RP62A; instead, many genetic studies have used strain 1457. Therefore, to better connect find

SUBMITTER: Yarawsky AE 

PROVIDER: S-EPMC11788477 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature