Metabolic consequences of erastin-induced ferroptosis in human ovarian cancer cells: an untargeted metabolomics study.

ABSTRACT:

Introduction

Ovarian cancer has been difficult to cure due to acquired or intrinsic resistance and therefore, newer or more effective drugs/approaches are needed for a successful treatment in the clinic. Erastin (ER), a ferroptosis inducer, kills tumor cells by generating and accumulating reactive oxygen species (ROS) within the cell, resulting in an iron-dependent oxidative damage-mediated ferroptotic cell death.

Methods

We have utilized human ovarian cancer cell lines, OVCAR-8 and its adriamycin-selected, multi-drug resistance protein (MDR1)-expressing NCI/ADR-RES, both equally sensitive to ER, to identify metabolic biomarkers of ferroptosis.

Results

Our studies showed that ER treatment rapidly depleted cellular glutathione and cysteine and enhanced formation of oph

SUBMITTER: Kirkwood-Donelson KI 

PROVIDER: S-EPMC11788483 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature