Probabilistic analysis of spatial viscoelastic cues in 3D cell culture using magnetic microrheometry.

ABSTRACT: Breast tumors are typically surrounded by extracellular matrix (ECM), which is heterogeneous, not just structurally but also mechanically. Conventional rheometry is inadequate for describing cell-size-level spatial differences in ECM mechanics that are evident at micrometer scales. Optical tweezers and passive microrheometry provide a microscale resolution for the purpose but are incapable of measuring ECM viscoelasticity (the liquid-like viscous and solid-like elastic characteristics) at stiffness levels as found in breast tumor biopsies. Magnetic microrheometry records data on varying microscale viscoelasticity within 3D ECM-mimicking materials up to the biopsy-relevant stiffness. However, the measurement probe-based microrheometry data has limitations in spatial resolution. Here, we pre

SUBMITTER: Arasalo O 

PROVIDER: S-EPMC11788488 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature