An optimized CTAB method for genomic DNA extraction from green seaweeds (Ulvophyceae).

ABSTRACT:

Premise

Seaweeds are gaining substantial research interest, particularly for genomic applications, where high-quality DNA is a prerequisite. Extracting DNA from these organisms presents challenges due to high levels of biomacromolecules resulting from their diverse cell structures. Existing protocols often lack versatility, leading to inconsistent outcomes across various materials and taxa, which highlights the need for a universal method for use with a variety of green seaweed samples.

Methods and results

We optimized the conventional cetyltrimethylammonium bromide (CTAB) protocol for green seaweed DNA extraction. Our method, involving an initial sample treatment, lysis buffer adjustment, and enzyme incubation alterations, outperformed the conventional CTAB and commercial k

SUBMITTER: Hossen R 

PROVIDER: S-EPMC11788905 | biostudies-literature | 2025 Jan-Feb

REPOSITORIES: biostudies-literature