The sensitivity of reconstructed carbon dioxide concentrations to stomatal preparation methods using a leaf gas exchange model.

ABSTRACT:

Premise

Mechanistic models using stomatal traits and leaf carbon isotope ratios to reconstruct atmospheric carbon dioxide (CO₂) concentrations (c _a ) are important to understand the Phanerozoic paleoclimate. However, methods for preparing leaf cuticles to measure stomatal traits have not been standardized.

Methods

Three people measured the stomatal density and index, guard cell length, guard cell pair width, and pore length of leaves from the same Ginkgo biloba, Quercus alba, and Zingiber mioga leaves growing at known CO₂ levels using four preparation methods: fluorescence on cleared leaves, nail polish, dental putty on fresh leaves, and dental putty on dried leaves.

Results

There are significant diff

SUBMITTER: Machesky MD 

PROVIDER: S-EPMC11788908 | biostudies-literature | 2025 Jan-Feb

REPOSITORIES: biostudies-literature