Retrospective SARS-CoV-2 human antibody development trajectories are largely sparse and permissive.

ABSTRACT: Immunological interventions, like vaccinations, are enabled by the predictive control of humoral responses to novel antigens. While the development trajectories for many broadly neutralizing antibodies (bnAbs) have been measured, it is less established how human subtype-specific antibodies develop from their precursors. In this work, we evaluated the retrospective development trajectories for eight anti-SARS-CoV-2 Spike human antibodies (Abs). To mimic the immunological process of BCR selection during affinity maturation in germinal centers (GCs), we performed deep mutational scanning on anti-S1 molecular Fabs using yeast display coupled to fluorescence-activated cell sorting. Focusing only on changes in affinity upon mutation, we found that human Ab development pathways have few mutations

SUBMITTER: Kirby MB 

PROVIDER: S-EPMC11789010 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature