Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-Down Proteomics.

ABSTRACT: The dynamic range challenge for the detection of proteins and their proteoforms in human plasma has been well documented. Here, we use the nanoparticle protein corona approach to enrich low-abundance proteins selectively and reproducibly from human plasma and use top-down proteomics to quantify differential enrichment for the 2841 detected proteoforms from 114 proteins. Furthermore, nanoparticle enrichment allowed top-down detection of proteoforms between ∼1 μg/mL and ∼10 pg/mL in absolute abundance, providing up to a 10⁵-fold increase in proteome depth over neat plasma in which only proteoforms from abundant proteins (>1 μg/mL) were detected. The ability to monitor medium and some low-abundant proteoforms through reproducible enrichment significantly extends the applicability o

SUBMITTER: Huang CF 

PROVIDER: S-EPMC11789057 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature