Dynamic changes of extracellular vesicles during zebrafish organogenesis.

ABSTRACT: Extracellular Vesicles (EVs) play a crucial role in cell differentiation. Despite its role as a well-established vertebrate model, little is known about EVs during zebrafish embryogenesis. This study investigates EVs during zebrafish embryogenesis, analysing size- and concentration-changes over time.Wild-type AB strain zebrafish larvae (zfl) were collected at 24, 48, 72, and 96 h post fertilization (hpf) and homogenized. EVs were isolated and characterized using flow cytometry, negative staining transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and Western Blot.Flow cytometry and TEM showed a high purity of the samples. Small EVs (sEVs) and large EVs (lEVs) were differentiated using NTA, showing that zfl use different types of EVs during embryogenesis. It was ob

SUBMITTER: Mulzer LM 

PROVIDER: S-EPMC11789338 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature