Forward programming of hPSCs to neutrophils using chemically defined media.

ABSTRACT: Polymorphonuclear neutrophils (PMNs), the most abundant leukocytes circulating in human blood, are pivotal players in the innate immune system. In recent years, PMNs have gained increasing recognition for their significant involvement in the pathogenesis of a wide array of human diseases, including sepsis, pulmonary conditions, autoimmune disorders, and various cancers. Due to their terminally differentiated state, PMNs possess a short lifespan and exhibit limited proliferative potential, which makes continuous replenishment from the bone marrow essential for maintaining immune homeostasis. This demand underscores the need for efficient, reliable, and robust methods of PMN production. In this study, we evaluated three forward programming protocols and one directed differentiation protocol

SUBMITTER: Hall HE 

PROVIDER: S-EPMC11789356 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature