Co-Translational Deposition of N⁶-Acetyl-L-Lysine in Nascent Proteins Contributes to the Acetylome in Mammalian Cells.

ABSTRACT: N⁶-acetyl-L-lysine residue is abundant in dietary protein but little is known about its potential influences on the diet-consumers. Herein, it is reported that Lysyl-tRNA synthetase (KARS) mediates co-translational deposition of diet-derived N⁶-acetyl-L-lysine (AcK) in nascent proteins to contribute to the acetylome in cells. Acetylated dietary protein is a direct source of AcK that can widely and substantially regulate the acetylome in multiple organs of mice. By analyzing the mechanisms underlying AcK contributing to the acetylome in mammalian cells, it is found that KARS can utilize AcK as an alternative substrate to produce N⁶-acetyl-l-lysyl-tRNA. The crystal structure of KARS in complex with AcK at 2.26 Å resolution shows that AcK shares the same subst

SUBMITTER: Guo D 

PROVIDER: S-EPMC11789599 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature