KAS-ATAC reveals the genome-wide single-stranded accessible chromatin landscape of the human genome.

ABSTRACT: Gene regulation in most eukaryotes involves two fundamental processes: alterations in genome packaging by nucleosomes, with active cis-regulatory elements (CREs) generally characterized by open-chromatin configuration, and transcriptional activation. Mapping these physical properties and biochemical activities, through profiling chromatin accessibility and active transcription, is a key tool for understanding the logic and mechanisms of transcription and its regulation. However, the relationship between these two states has not been accessible to simultaneous measurement. To this end, we developed KAS-ATAC, a combination of the kethoxal-assisted ssDNA sequencing (KAS-seq) and assay for transposase-accessible chromatin using sequencing (ATAC-seq) methods for mapping single-stranded D

SUBMITTER: Kim SH 

PROVIDER: S-EPMC11789636 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature