LC-MS/MS of isomeric N-and O-glycopeptides on mesoporous graphitized carbon column.

ABSTRACT:

Background

The study of glycopeptides is associated with challenges regarding the microheterogeneity of different isomeric glycans occupying the same glycosylation sites in glycoproteins. It is immensely valuable to perform both qualitative and quantitative site-specific glycosylation analysis of glycopeptide isomers due to their link to several diseases. Achieving isomeric separation of glycopeptides is particularly challenging due to the low abundance of glycopeptides as well as inefficient ionization. Although some methods have demonstrated the isomeric separation of glycopeptides, a more efficient nanoflow-based stationary phase is needed for the isomeric separation of both N- and O-glycopeptides.

Results

In this study, the separation of N- and O-glycopeptide isomers at

SUBMITTER: Daramola O 

PROVIDER: S-EPMC11789930 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature