Whole-genome analysis of a ST45-SCC<i>mec</i> IVa (2B)-t116 methicillin-resistant <i>Staphylococcus aureus</i> strain isolated from the sputum of a 5-year-old child with pneumonia.

ABSTRACT:

Introduction

Methicillin-resistant Staphylococcus aureus (MRSA) sequence type (ST) 45 is a major global MRSA lineage with huge strain diversity and a high clinical impact. In Hainan and Guangzhou of China, the ST45-MRSA was mainly associated with t116.

Methods

The MRSA strain SA2107 was isolated from the sputum of a 5-year-old child with pneumonia. The whole genome of SA2107 was sequence using Illumina (Novaseq 6000) and PacBio (Sequel IIe) sequencers, and the sequences were assembled using hybrid assembly. The carriage of antibiotic resistance genes, virulence genes, and mobile genetic elements were identified using bioinformatics tools. The comparative genomic analyses of MRSA strain SA2107 with other MRSA strains worldwide were performed.

Findings

The genome

SUBMITTER: Huang L 

PROVIDER: S-EPMC11790441 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature